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Sequencing
Simplified

Microbes

Agriculture

Eukaryotes

Human

Environmental

SeqCenter has been delivering genomics at scale since 2019. Founded by evolutionary biologists, our sequencing and analysis pipelines focus on guaranteed rapid turnaround (≤2 weeks) and sample input flexibility.

Our microbial sequencing workflows are optimized for isolate-to-population scale studies, enabling high-contiguity assemblies, precise strain differentiation, and reproducible variant detection. By integrating short- and long-read technologies, we support everything from rapid pathogen characterization to large comparative genomics projects. With deep experience in bacterial, viral, and fungal systems, we deliver data quality that meets publication and regulatory standards without sacrificing speed.

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SeqCenter has been delivering genomics at scale since 2019. Founded by evolutionary biologists, our sequencing and analysis pipelines focus on guaranteed rapid turnaround (≤2 weeks) and sample input flexibility.

For agricultural genomics, our pipelines are designed to balance scale, cost, and biological complexity across plant, animal, and microbial systems. We enable accurate variant discovery, structural variation analysis, and transcriptomic profiling to support breeding programs, trait mapping, and production optimization. Our sequencing strategies are tailored to large genomes and high sample throughput, ensuring consistent, actionable results aligned with project timelines.

Recommended Services

SeqCenter has been delivering genomics at scale since 2019. Founded by evolutionary biologists, our sequencing and analysis pipelines focus on guaranteed rapid turnaround (≤2 weeks) and sample input flexibility.

Eukaryotic projects require careful handling of genome complexity, repetitive content, and transcript diversity. Our multi-platform approach combines deep coverage of >Q30 data with long-read sequencing to resolve structural variation, improve assembly contiguity, and capture full-length isoforms. The result is a streamlined path to high-quality genomes and transcriptomes suitable for downstream functional and comparative analyses.

Recommended Services

SeqCenter has been delivering genomics at scale since 2019. Founded by evolutionary biologists, our sequencing and analysis pipelines focus on guaranteed rapid turnaround (≤2 weeks) and sample input flexibility.

Our human genomics workflows prioritize sensitivity, reproducibility, and data integrity, supporting both research and translational applications. From cohort-scale sequencing to targeted studies, we deliver high-depth, high-confidence data for variant detection and gene expression analysis. Rigorous QC, rapid turnaround, and flexible project design ensure your study progresses efficiently while meeting the highest scientific standards.

Recommended Services

SeqCenter has been delivering genomics at scale since 2019. Founded by evolutionary biologists, our sequencing and analysis pipelines focus on guaranteed rapid turnaround (≤2 weeks) and sample input flexibility.

Environmental samples present unique challenges due to complexity, low input, and community diversity. Our workflows are built to provide robust metagenomic and amplicon sequencing solutions that deliver accurate taxonomic and functional insights across diverse ecosystems. Whether profiling microbial communities or exploring ecosystem dynamics, our scalable pipelines enable rapid, reliable data generation from even the most heterogeneous samples.

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Our Services

We are proud to offer a wide range of sequencing services across Illumina, Oxford Nanopore, and PacBio platforms, all with our two-week turnaround guarantee. From single samples to large-scale projects, SeqCenter is your sequencing partner. Explore our services below to see how SeqCenter can enable and expedite your research. For services beyond our standard offerings, please contact us to discuss your research goals.

  • DNA Icon

    Illumina DNA Sequencing

  • DNA Icon

    Illumina Protein Prep

  • DNA Icon

    Extraction Services

  • RNA Icon

    Illumina RNA Sequencing

  • PacBio Icon

    PacBio Sequencing

  • ONT Ligation Icon

    Nanopore Sequencing

  • Metagenome Icon

    Microbiome Sequencing

  • SARS-COV icon

    SARS-CoV-2 Sequencing

  • Flowcell Icon

    Illumina Full Flowcell Runs

  • Bioinformatics icon

    Bioinformatics

Our Ordering Process

Simplicity and speed are hallmarks of SeqCenter’s business model. Our entire ordering process from quote generation through checkout can be completed online in a few minutes. However, if you need assistance, we’re always here to help.

Obtain a Quote or Place an Order

Fill out our easy-to-use form, receive your quote, and finalize your order.

Submit Your Samples

Follow our simple directions and ship your samples to our Pittsburgh address.

Receive Your Data

Within 14 days – most times even less than that – we’ll upload your data to a personal folder for you to access.

Our Recent Blogs

Client Reviews

Michael Gray headshot

Michael J. Gray, M.S., Ph.D.

Department of Microbiology

The University of Alabama at Birmingham

SeqCenter is a pleasure to work with, and we’ve been very happy with the quality of their results and service. We sequence every bacterial strain we make now, and it’s noticeably improved the rigor of all our experiments.

Nancy Hanson headshot

Nancy D. Hanson, Ph.D.

Center for Research in Anti-Infectives and Biotechnology

Creighton University Medical School

We have requested multiple analyses on data sets and couldn’t be more pleased with their ability to deliver what we requested. They are always willing to answer questions about what we might need for our analyses and the turnaround time is fantastic. We sent over 130 samples once, and they still sent the data within two weeks. Remarkable. We won’t go anywhere else.

Kenneth Day headshot

Kenny Day

Head of Research and Development

Vidium Animal Health

SeqCenter in Pittsburgh is honestly the best sequencing service center I have worked with so far in my career since starting in NGS around 2009. We started working with SeqCenter in Spring 2023 and are very impressed. The ease of ordering on their website, the superb turnaround time, quality of results, straightforward fastq data transfer, and expertise of the staff are just a few reasons for strongly recommending this team to entrust your library with. Most of all, SeqCenter carries a personalized customer service approach that goes above and beyond at a high level of professionalism.

Our Accolades

Join the global community of researchers who embrace the limitless possibilities of our sequencing services. Elevate your sequencing needs with our unparalleled speed and service.

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Contact:
91 43rd Street, Ste. 250
Pittsburgh, PA 15201
(878) 227-4915

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Full Flowcell:

We offer full flowcell sequencing services for libraries that have been prepared outside of our lab. For each library, we will need to know the loading concentration, phiX spike-in, and run structure. Data is delivered as a complete run folder and can be demultiplexed upon request if a sample sheet is provided.

SARS-CoV-2 Sequencing:

Our SARS-CoV-2 Sequencing services include an assembly on the SARS-CoV-2 reference genome as a scaffold. These packages provide roughly 200Mbp of paired end reads (2×151 bp) as fastq files and the assembly in fasta format.

We require a minimum sample volume of 15 µL of extracted material (or 250µL for VTM samples) and recommend that only samples with Ct values below 35 are sent (because submissions are not measured for viral load before processing). We offer services for both purified RNA and samples in VTM media that require viral RNA extraction. Please see our SARS-CoV-2 Sequencing page for more detailed information.

Microbiome Sequencing:

Please see our Microbiome Sequencing page for package details, including distributables and sample requirements.

Nanopore Sequencing:

Our Oxford Nanopore sequencing packages provide reads equal to the length of input DNA, delivered as a fastq file.

For each sample, we require a minimum volume of 60 µL, and at least 40 ng/µL dsDNA quantified by Qubit or other dye-based method (100 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.

Please see our DNA Sequencing: Long Read Sequencing page for more detailed information about the package, variant calling analysis, and extraction requirements.

PacBio:

Our PacBio services deliver HiFi reads with an average read length of 8-12kb for standard offerings and up to 25kb for full flowcell runs. All package names are listed by the minimum number of >Q20 reads that will be delivered. Both assembly and annotation are included with all packages.

For each sample, a minimum volume of 60 µL and at least 35 ng/µL dsDNA (quantified by Qubit or other dye-based methods) are required. SeqCenter strongly recommends utilizing our HMW DNA Extraction service for best results; however, we will also accept samples that are shipped on dry ice to avoid DNA integrity issues caused by the shipping process.

Please see our PacBio Sequencing page for more detailed information about our services.

RNA Sequencing:

We offer both rRNA depletion RNA squencing and polyA-tail enrichment mRNA sequencing. Both provide stranded, paired end reads (2x 151bp) as fastq files.

For each sample, we require a minimum volume of 20µL and at least 50ng/µL RNA. We also recommend, but do not require, that samples be DNAse treated and have a RIN score greater than 6. We also offer Basic and Intermediate RNAseq analyses, which require an annotated reference sequence.

Please see our RNA Sequencing page for more detailed information about both our RNA sequencing and analysis packages.

Illumina Sequencing:

The Illumina sequencing packages produce paired end reads (2×151 bp) delivered as fastq files.

For each sample, we require a minimum volume of 30 µL and at least 10 ng/µL dsDNA quantified by Qubit or other dye-based method (20 ng/µL by Nanodrop). Variant calling add-on analysis requires an annotated reference file. DNA extraction services are available for only specific categories of microbes.

Please see our DNA Sequencing: Whole Genome Sequencing page for more detailed information about the package, variant calling analysis, and extraction requirements.

For additional information about variant calling and short read assemblies, please see our Bioinformatics page.

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